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disA [2019-08-16 10:23:38]

DNA integrity scanning protein, diadenylate cyclase, delays [SW|sporulation] in the case of chromosome damage, the DisA-dependent checkpoint arrests [SW|DNA replication] during B. subtilis spore outgrowth until the germinating spores genome is free of damage

locus

BSU_00880

5.57

40.58 kDa

protein length

360 aa Sequence Blast

gene length

1083 bp Sequence Blast

function

control of [SW|sporulation] initiation

product

DNA integrity scanning protein, has diadenylate cyclase activity

essential

2.7.7.85

synonyms

yacK

Outlinks

KEGG BsubCyc SubtiList UniProt Expression Browser

Genomic Context

Hide small RNAs Zoom:

categories

[category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.8|Cell division] → [category|SW 1.1.8.2|Other genes]

[category|SW 2|Metabolism] → [category|SW 2.5|Nucleotide metabolism] → [category|SW 2.5.3|Metabolism of signalling nucleotides]

[category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.3|Sporulation/ other]

[category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.1|General stress proteins (controlled by SigB)]

[category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.2|Cell envelope stress proteins (controlled by SigM, V, W, X, Y)]

[category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.4|Heat shock proteins]

Gene

Coordinates

107,476 108,558

Phenotypes of a mutant

reduced spore survival after infrared exposure [pubmed|28961460]

altered morphology on MSgg medium [pubmed|29588402]

plant root colonization defect [pubmed|29588402]

The protein

Catalyzed reaction/ biological activity

synthesis of c-di-AMP from two molecules of ATP [Pubmed|23192352,21566650]

binds [protein|A44D4677FB70BE8F554BF1001A500F817C7DA95F|RecA] to inhibit its activity [pubmed|30916351]

2 ATP --> c-di-AMP + 2 diphosphate (according to UniProt)

Protein family

DisA family (single member, according to UniProt)

[SW|Domains]

contains a [SW|DAC domain] for the synthesis of c-di-AMP [Pubmed|18439896]

[SW|Cofactors]

Mn²⁺ [pubmed|26014055]

Effectors of protein activity

the presence of potassium results in enhanced activity [pubmed|28420751]

Structure

[PDB|3C23] [Pubmed|18439896]

[SW|Localization]

see a [http://download.cell.com/mmcs/journals/0092-8674/PIIS0092867406005034.mmc12.avi video] showing the movement of DisA in the cell (in real time) [Pubmed|16713562]

forms discrete globular foci in germinating spres that colocalize with the nucleoid [Pubmed|24244006]

forms rapidly moving focus that pausesat [protein|A44D4677FB70BE8F554BF1001A500F817C7DA95F|RecA]-mediated recombination intermediates upon induction of DNA damage during spore development [pubmed|30916351]

additional information

subject to Clp-dependent proteolysis upon glucose starvation [pubmed|17981983]

Expression and Regulation

Operons

genes

description

[Pubmed|8793870]

sigma factors

regulatory mechanism

regulation

expressed during germination and spore outgrowth [Pubmed|24244006]

induction during diamide stress ([protein|2C6386E9A63F410558D168798D077DF91590F454|Spx]) [pubmed|30962353]

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genes

[gene|2B091CCEE9E34D659771E39B1FC9050A145048AB|disA]

description

[Pubmed|17434969]

regulation

expressed during germination and spore outgrowth [Pubmed|24244006]

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Biological materials

Mutant

MGNA-B932 ([gene|2B091CCEE9E34D659771E39B1FC9050A145048AB|disA]::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1931 NBRP B. subtilis, Japan]

GP2142 (''Δ''''[gene|2B091CCEE9E34D659771E39B1FC9050A145048AB|disA]''::''tet''), available in [SW|Jörg Stülke]'s lab

1A939 ( ''disA''::''tet''), [Pubmed|16713562], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A939&Search=1A939 BGSC]

BKE00880 ([gene|2B091CCEE9E34D659771E39B1FC9050A145048AB|disA]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE00880 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CCCTTTTTTCTCTTTTTCCA, downstream forward: _UP4_TGATTTCGGTTAAAACCTTA

BKK00880 ([gene|2B091CCEE9E34D659771E39B1FC9050A145048AB|disA]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK00880 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CCCTTTTTTCTCTTTTTCCA, downstream forward: _UP4_TGATTTCGGTTAAAACCTTA

GP2715 (''Δ''''[gene|2B091CCEE9E34D659771E39B1FC9050A145048AB|disA]''::''spc''), available in [SW|Jörg Stülke]'s lab

GP2752 (''Δ''''[gene|2B091CCEE9E34D659771E39B1FC9050A145048AB|disA]''::''cat''), available in [SW|Jörg Stülke]'s lab

GP2782 (''Δ''''[gene|2B091CCEE9E34D659771E39B1FC9050A145048AB|disA]''::''kan''), available in [SW|Jörg Stülke]'s lab

Expression vectors

IPTG inducible expression of His-''disA'' in ''E. coli'': pGP2563 (in [http://biochem.web.utah.edu/hill/links/pET19b.pdf pET19b]), available in [SW|Jörg Stülke]'s lab

two-hybrid system

B. pertussis adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Fabian Commichau]'s lab

References

Reviews

22933559,18714086,25869574,23812326,30224435

Original publications

24244006,8793870,9987115,16713562,23760274,11544224,17434969,18439896,16713555,12493822,21566650,23192352,23608499,25616256,26014055,27150552,28420751,28511132,28961460,29536659,29588402,30877841,30916351